Hotspot

psbM-trnD-GUC

LSC · 29658-30778 · 1121 bp

Back to Bistorta

Pi 0.022

MarkerSeek score 63.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1121
variable_sites: 61
pi: 0.022
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.2	58.5	937-1232 bp	1.0	62.0
psbM-trnD-GUC_p2	ACTAGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	43.5	50.0	58.0	58.5	940-1235 bp	1.0	60.5
psbM-trnD-GUC_p3	AGGAACTAGAATGAAGAGTGCAG	TTCAATTGGTCAGAGCACCG	43.5	50.0	57.9	58.5	944-1239 bp	1.0	59.8
psbM-trnD-GUC_p4	AGGAACTAGAATGAAGAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	56.8	58.5	944-1239 bp	1.0	59.2
psbM-trnD-GUC_p5	GGAACTAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	43.5	50.0	58.2	58.5	943-1238 bp	1.0	59.1