Hotspot

trnC-GCA-petN

LSC · 27796-28596 · 801 bp

Back to Bistorta

Pi 0.026

MarkerSeek score 68.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 801
variable_sites: 52
pi: 0.026
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCGGTTCAAATCTGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	850-887 bp	1.0	83.8
trnC-GCA-petN_p2	TTTCCCCGGTTCAAATCTGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	911-948 bp	1.0	81.8
trnC-GCA-petN_p3	TTTCCCCGGTTCAAATCTGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	849-886 bp	1.0	77.8
trnC-GCA-petN_p4	CCGGTTCAAATCTGGGTGTC	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.8	58.2	845-882 bp	1.0	77.8
trnC-GCA-petN_p5	TTTCCCCGGTTCAAATCTGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.1	58.9	858-895 bp	1.0	77.7