Hotspot

atpH-atpI

LSC · 13462-14171 · 710 bp

Back to Bistorta

Pi 0.0232

MarkerSeek score 63.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 710
variable_sites: 43
pi: 0.0232
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TACCTTCTACAGCTTGGCCT	TATCGTCCTTGGGTTCCCTT	50.0	50.0	58.0	58.0	1267-1303 bp	1.0	85.1
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CAAGCGGTATTCAGGCTCTT	45.0	50.0	58.0	58.0	819 bp	0.222222	48.5
atpH-atpI_p3	TACCTTCTACAGCTTGGCCT	CAAGCGGTATTCAGGCTCTT	50.0	50.0	58.0	58.0	902 bp	0.222222	48.4
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	TATCGTCCTTGGGTTCCCTT	45.0	50.0	58.0	58.0	1216 bp	0.222222	48.4
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	GCCGCACCTACGAATGATAT	45.0	50.0	58.0	57.9	1105 bp	0.222222	48.1