Hotspot

psbI-trnS-GCU

LSC · 7682-7781 · 100 bp

Back to Bistorta

Pi 0.0473

MarkerSeek score 65.80

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 100
variable_sites: 11
pi: 0.0473
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	AAAGGGAGGCTCCTAACACT	GTCATTCGTACCGAGGGTTC	50.0	55.0	58.0	58.1	389-391 bp	0.222222	48.5
psbI-trnS-GCU_p2	AAGGGAGGCTCCTAACACTT	GTCATTCGTACCGAGGGTTC	50.0	55.0	58.0	58.1	388-390 bp	0.222222	48.5
psbI-trnS-GCU_p3	AAAGGGAGGCTCCTAACACT	TAGGGAGAGATGGCTGAGTG	50.0	55.0	58.0	57.9	443-445 bp	0.222222	48.4
psbI-trnS-GCU_p4	AAGGGAGGCTCCTAACACTT	TAGGGAGAGATGGCTGAGTG	50.0	55.0	58.0	57.9	442-444 bp	0.222222	48.4
psbI-trnS-GCU_p5	AAGAGAAAAGGGAGGCTCCT	TAGGGAGAGATGGCTGAGTG	50.0	55.0	57.9	57.9	449-451 bp	0.222222	48.4