Hotspot

trnK-UUU-rps16

LSC · 4134-4900 · 767 bp

Back to Bistorta

Pi 0.0266

MarkerSeek score 68.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 767
variable_sites: 50
pi: 0.0266
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	AAAAGGGTATGTTGCTGCCA	55.0	45.0	58.0	58.0	1601-1651 bp	1.0	86.9
trnK-UUU-rps16_p2	CGAATCCCTTGCTTCATCCA	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.0	58.0	1554-1604 bp	1.0	86.9
trnK-UUU-rps16_p3	ACGAATCCCTTGCTTCATCC	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.0	58.0	1555-1605 bp	1.0	86.9
trnK-UUU-rps16_p4	GGACGAATCCCTTGCTTCAT	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.0	58.0	1557-1607 bp	1.0	86.9
trnK-UUU-rps16_p5	TCAGGATCAGTCGTGGTCTT	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.1	58.0	1597-1647 bp	1.0	86.5