Hotspot

ycf1

SSC · 123604-128802 · 5199 bp

Back to Biscutella

Pi 0.0594

MarkerSeek score 74.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5199
variable_sites: 548
pi: 0.0594
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGAGGCCCAAATGGTAGTTT	TGATTTTCGTCGAGCGCTTA	50.0	45.0	57.8	58.0	687 bp	0.25	48.0
ycf1_p2	TTCTGTTTCGGCTAGTGCAA	TGATTTTCGTCGAGCGCTTA	45.0	45.0	57.8	58.0	493 bp	0.25	47.9
ycf1_p3	GGAGGCCCAAATGGTAGTTT	GGATCTCTCGCGGAAGAATC	50.0	55.0	57.8	57.9	1246 bp	0.25	47.7
ycf1_p4	GCATTTCACGTTCCCAATCC	TGATTTTCGTCGAGCGCTTA	50.0	45.0	57.7	58.0	449 bp	0.25	47.7
ycf1_p5	TTCTGTTTCGGCTAGTGCAA	GGATCTCTCGCGGAAGAATC	45.0	55.0	57.8	57.9	1052 bp	0.25	47.5