Hotspot

rpl32-trnL

SSC · 113116-114072 · 957 bp

Back to Biscutella

Pi 0.0507

MarkerSeek score 82.50

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 957
variable_sites: 84
pi: 0.0507
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	AGTGCTTGTCTTCCAGTACAC	TGCAGACTGAGGCATTTTGT	47.6	45.0	57.9	58.0	2049-2083 bp	1.0	79.4
rpl32-trnL_p2	GTGCTTGTCTTCCAGTACACT	TGCAGACTGAGGCATTTTGT	47.6	45.0	57.9	58.0	2048-2082 bp	1.0	79.4
rpl32-trnL_p3	AGGGAAATCACTTTCGACAGG	TGCAGACTGAGGCATTTTGT	47.6	45.0	57.9	58.0	1577-1597 bp	1.0	79.2
rpl32-trnL_p4	GTGCTTGTCTTCCAGTACACT	TAACCACTGGGACTGGAGAG	47.6	55.0	57.9	58.1	2081-2115 bp	1.0	78.9
rpl32-trnL_p5	AGGGAAATCACTTTCGACAGG	TAACCACTGGGACTGGAGAG	47.6	55.0	57.9	58.1	1610-1630 bp	1.0	78.7