Hotspot

ndhF-rpl32

SSC · 112046-112956 · 911 bp

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Pi 0.0475

MarkerSeek score 79.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 911
variable_sites: 72
pi: 0.0475
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AACAGGTCGTGTGAACCAAA	CCTGTCGAAAGTGATTTCCCT	45.0	47.6	57.9	57.9	1482-1543 bp	1.0	79.1
ndhF-rpl32_p2	ACAGGTCGTGTGAACCAAAA	CCTGTCGAAAGTGATTTCCCT	45.0	47.6	57.9	57.9	1481-1542 bp	1.0	79.1
ndhF-rpl32_p3	GGTCGTGTGAACCAAAAACC	CCTGTCGAAAGTGATTTCCCT	50.0	47.6	57.8	57.9	1478-1539 bp	1.0	78.5
ndhF-rpl32_p4	CATGAAGAGGAAATTGCGCG	CCTGTCGAAAGTGATTTCCCT	50.0	47.6	58.2	57.9	1714-1775 bp	1.0	78.3
ndhF-rpl32_p5	GAAATTGCGCGGATTTAGCA	CCTGTCGAAAGTGATTTCCCT	45.0	47.6	57.8	57.9	1705-1766 bp	1.0	78.3