Hotspot

atpH-atpI

LSC · 13374-13901 · 528 bp

Back to Biscutella

Pi 0.0414

MarkerSeek score 81.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 528
variable_sites: 38
pi: 0.0414
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TACAAGCGGGATTCAAGCTC	45.0	50.0	58.1	58.0	622-637 bp	1.0	87.8
atpH-atpI_p2	GTCCAATAGAAGCAAGCCCA	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	662-677 bp	1.0	87.3
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	680-695 bp	1.0	87.2
atpH-atpI_p4	GCTGTACCTTGACCAACTCC	TACAAGCGGGATTCAAGCTC	55.0	50.0	58.2	58.0	684-699 bp	1.0	86.5
atpH-atpI_p5	AACGGAAGCAGCAGAAATCA	GGCTGCGGCTTATATAGGTG	45.0	55.0	58.1	58.3	586-601 bp	1.0	86.1