Hotspot

trnT-trnR

LSC · 9246-9406 · 161 bp

Back to Biscutella

Pi 0.0549

MarkerSeek score 77.40

Primer available yes

Label trnT-trnR

Feature Metadata

label_name: trnT-trnR
feature_type: igs
region: LSC
length_bp: 161
variable_sites: 16
pi: 0.0549
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnR_p1	AGCCTTCCAAGCTAACGATG	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	283-290 bp	0.75	40.8
trnT-trnR_p2	ACCCTTAGCCTTCCAAGCTA	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	289-296 bp	0.75	40.7
trnT-trnR_p3	CGACGTCGACTAAAACCCTT	TGATATTGCGTCCAATAGGA	50.0	40.0	57.9	53.9	303-310 bp	0.75	40.3
trnT-trnR_p4	AAACCCTTAGCCTTCCAAGC	TGATATTGCGTCCAATAGGA	50.0	40.0	58.1	53.9	291-298 bp	0.75	40.3
trnT-trnR_p5	AGCCTTCCAAGCTAACGATG	TTGATATTGCGTCCAATAGGA	50.0	38.1	58.0	54.8	284-291 bp	0.75	39.8