Hotspot

trnH-psbA

LSC · 71-326 · 256 bp

Back to Biscutella

Pi 0.0964

MarkerSeek score 79.20

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 256
variable_sites: 44
pi: 0.0964
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	CCACTTGGCTACATCCGC	GAGGCTCCATCTACAAATGG	61.1	50.0	58.2	55.6	298-345 bp	1.0	55.2
trnH-psbA_p2	TCCACTTGGCTACATCCGC	GAGGCTCCATCTACAAATGG	57.9	50.0	59.8	55.6	299-346 bp	1.0	52.0
trnH-psbA_p3	TCCACTTGGCTACATCCG	GAGGCTCCATCTACAAATGG	55.6	50.0	56.3	55.6	299-346 bp	1.0	52.0
trnH-psbA_p4	CCACTTGGCTACATCCGC	AGGCTCCATCTACAAATGG	61.1	47.4	58.2	54.2	297-344 bp	1.0	52.0
trnH-psbA_p5	TCCACTTGGCTACATCCGC	AGGCTCCATCTACAAATGG	57.9	47.4	59.8	54.2	298-345 bp	1.0	52.0