Hotspot

rps16

LSC · 5166-6328 · 1163 bp

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Pi 0.00439
MarkerSeek score 40.90
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1163
variable_sites
44
pi
0.00439
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
40.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 AGCAAGAAAATAAGTAGAGCAAGAA GGTTCGAAATTAGAGGAATGT 32.0 38.1 57.0 53.6 1268-1302 bp 0.962963 43.2
rps16_p2 AGCAAGAAAATAAGTAGAGCAAGA GGTTCGAAATTAGAGGAATGT 33.3 38.1 56.4 53.6 1284-1302 bp 0.925926 40.2
rps16_p3 AGCAAGAAAATAAGTAGAGCAAGA GGTTCGAAATTAGAGGAATGTT 33.3 36.4 56.4 54.5 1284-1302 bp 0.925926 40.2
rps16_p4 GCAAGAAAATAAGTAGAGCAAGA GGTTCGAAATTAGAGGAATGT 34.8 38.1 55.0 53.6 1283-1301 bp 0.925926 40.2
rps16_p5 AGCAAGAAAATAAGTAGAGCAAG GGTTCGAAATTAGAGGAATGT 34.8 38.1 55.0 53.6 1284-1302 bp 0.925926 40.2