Hotspot

ndhF-ycf1

SSC · 126933-126974 · 42 bp

Back to Bidens

Pi 0.0465
MarkerSeek score 65.40
Primer available yes
Label ndhF-ycf1

Feature Metadata

label_name
ndhF-ycf1
feature_type
igs
region
SSC
length_bp
42
variable_sites
18
pi
0.0465
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-ycf1_p1 GGGTGGACGCATTTCTTCTT GTTGGTTGGATAATTGGTCACA 50.0 40.9 58.5 56.9 173-243 bp 1.0 60.0
ndhF-ycf1_p2 GGGTGGACGCATTTCTTCTT TGTTGGTTGGATAATTGGTCAC 50.0 40.9 58.5 56.9 174-244 bp 1.0 60.0
ndhF-ycf1_p3 GGGTGGACGCATTTCTTCTT TTGTTGGTTGGATAATTGGTCAC 50.0 39.1 58.5 57.5 175-245 bp 1.0 57.3
ndhF-ycf1_p4 GGGTGGACGCATTTCTTCTT TGGTTGGATAATTGGTCACA 50.0 40.0 58.5 54.5 171-241 bp 1.0 57.2
ndhF-ycf1_p5 GGGTGGACGCATTTCTTCTT TGTTGGTTGGATAATTGGTCACA 50.0 39.1 58.5 58.5 174-244 bp 1.0 56.8