Hotspot

rrn5-trnR-ACG

IRb · 106780-107062 · 283 bp

Back to Bidens

Pi 0.0196
MarkerSeek score 58.40
Primer available yes
Label rrn5-trnR-ACG

Feature Metadata

label_name
rrn5-trnR-ACG
feature_type
igs
region
IRb
length_bp
283
variable_sites
17
pi
0.0196
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.91
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn5-trnR-ACG_p1 GGTGACGATACTGTAGGGGA CCACGTGCTCTAATCCTCTG 55.0 55.0 57.9 57.8 340-368 bp 1.0 85.7
rrn5-trnR-ACG_p2 ATTCTGGTGTCCTAGGCGTA CCACGTGCTCTAATCCTCTG 50.0 55.0 57.8 57.8 408-436 bp 1.0 84.3
rrn5-trnR-ACG_p3 GTAGAGGAACCACACCAACC CCACGTGCTCTAATCCTCTG 55.0 55.0 57.8 57.8 391-419 bp 1.0 84.3
rrn5-trnR-ACG_p4 CGTAGAGGAACCACACCAAC CCACGTGCTCTAATCCTCTG 55.0 55.0 58.2 57.8 392-420 bp 1.0 84.0
rrn5-trnR-ACG_p5 TGTCCTAGGCGTAGAGGAAC CCACGTGCTCTAATCCTCTG 55.0 55.0 58.2 57.8 401-429 bp 1.0 83.7