Hotspot

rpl16-rps3

LSC · 81316-82531 · 1216 bp

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Pi 0.00576
MarkerSeek score 55.60
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
1216
variable_sites
61
pi
0.00576
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.97
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 CAAGTGCCTGAAGAGCGTAT GAGGGTAGGGTTCCACTACA 50.0 55.0 58.0 57.8 1409-1448 bp 0.962963 82.3
rpl16-rps3_p2 GGGTTCAAGTGCCTGAAGAG GAGGGTAGGGTTCCACTACA 55.0 55.0 58.5 57.8 1414-1453 bp 1.0 81.7
rpl16-rps3_p3 CAAGTGCCTGAAGAGCGTAT AGAGGGTAGGGTTCCACTAC 50.0 55.0 58.0 57.5 1410-1449 bp 0.962963 79.9
rpl16-rps3_p4 CAAGTGCCTGAAGAGCGTAT AGGGTAGGGTTCCACTACAA 50.0 50.0 58.0 57.3 1408-1447 bp 0.962963 78.4
rpl16-rps3_p5 CAAGTGCCTGAAGAGCGTAT GAGAGGGTAGGGTTCCACTA 50.0 55.0 58.0 57.2 1411-1450 bp 0.962963 77.5