Hotspot

petA-psbJ

LSC · 62660-63455 · 796 bp

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Pi 0.00824
MarkerSeek score 65.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
796
variable_sites
59
pi
0.00824
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ACGTGTACAAGGCCTTTTGT GGTTCATATTCCGGGTTGGG 45.0 55.0 58.0 58.3 929-963 bp 1.0 84.5
petA-psbJ_p2 GGCCTTTTGTTCTTCTTCGC GGTTCATATTCCGGGTTGGG 50.0 55.0 57.9 58.3 919-953 bp 1.0 84.5
petA-psbJ_p3 ACGTGTACAAGGCCTTTTGT CATATTCCGGGTTGGGTTCA 45.0 50.0 58.0 57.6 925-959 bp 1.0 83.6
petA-psbJ_p4 ACGTGTACAAGGCCTTTTGT TGGTTCATATTCCGGGTTGG 45.0 50.0 58.0 57.6 930-964 bp 1.0 83.6
petA-psbJ_p5 ACGTGTACAAGGCCTTTTGT GTTCATATTCCGGGTTGGGT 45.0 50.0 58.0 57.6 928-962 bp 1.0 83.6