Hotspot

accD-psaI

LSC · 58101-58829 · 729 bp

Back to Bidens

Pi 0.00648
MarkerSeek score 56.60
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
729
variable_sites
43
pi
0.00648
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.98
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TTCCAGAAGGGCTTATTCGA AAAGAAGCCATTGCGATTGC 45.0 45.0 56.2 58.0 891-915 bp 1.0 73.0
accD-psaI_p2 TTCCAGAAGGGCTTATTCGA GCGATTGCCGGAAAAACTAG 45.0 50.0 56.2 57.8 879-903 bp 1.0 71.7
accD-psaI_p3 TCCAGAAGGGCTTATTCGAT AAAGAAGCCATTGCGATTGC 45.0 45.0 56.0 58.0 890-914 bp 1.0 71.1
accD-psaI_p4 TTCCAGAAGGGCTTATTCGA AGGCCTACTAAAGGGACCAA 45.0 50.0 56.2 57.6 861-885 bp 1.0 70.3
accD-psaI_p5 GCGGCTGAGTATTTATTCCAG AAAGAAGCCATTGCGATTGC 47.6 45.0 56.8 58.0 906-930 bp 1.0 69.7