Hotspot

trnY-GUA-rpoB

LSC · 11756-12842 · 1087 bp

Back to Bidens

Pi 0.00414
MarkerSeek score 57.10
Primer available yes
Label trnY-GUA-rpoB

Feature Metadata

label_name
trnY-GUA-rpoB
feature_type
igs
region
LSC
length_bp
1087
variable_sites
35
pi
0.00414
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.95
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-rpoB_p1 CGAATTTACAGTCCGTCCCC AGAAAGCTCCTCTGCCAAAC 55.0 50.0 58.4 58.1 1152-1214 bp 1.0 82.8
trnY-GUA-rpoB_p2 ACGAATTTACAGTCCGTCCC AGAAAGCTCCTCTGCCAAAC 50.0 50.0 57.6 58.1 1153-1215 bp 1.0 82.7
trnY-GUA-rpoB_p3 CGAATTTACAGTCCGTCCCC CTCTGCCAAACCCTGATCAA 55.0 50.0 58.4 57.8 1143-1205 bp 1.0 82.0
trnY-GUA-rpoB_p4 ATTGACAAATTGTTGGGCCG AGAAAGCTCCTCTGCCAAAC 45.0 50.0 57.5 58.1 1206-1268 bp 1.0 81.9
trnY-GUA-rpoB_p5 ACGAATTTACAGTCCGTCCC CTCTGCCAAACCCTGATCAA 50.0 50.0 57.6 57.8 1144-1206 bp 1.0 81.8