Hotspot

psbA-trnK-UUU

LSC · 1545-1748 · 204 bp

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Pi 0.011
MarkerSeek score 58.30
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
204
variable_sites
25
pi
0.011
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.94
markerseek_score
58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 ACGCCAAACCATCCAATGTA CTAGTTCCGGGTTCGAATCC 45.0 55.0 57.8 57.8 326-339 bp 1.0 86.9
psbA-trnK-UUU_p2 TAAAGACGGTTTTCGGTGCT GAAAGCCGTGTGCAATGAAA 45.0 45.0 57.8 57.9 393-406 bp 1.0 86.3
psbA-trnK-UUU_p3 ACGCCAAACCATCCAATGTA GAAAGCCGTGTGCAATGAAA 45.0 45.0 57.8 57.9 411-424 bp 1.0 86.2
psbA-trnK-UUU_p4 AGGGATCATCAAAACGCCAA GAAAGCCGTGTGCAATGAAA 45.0 45.0 57.8 57.9 424-437 bp 1.0 85.9
psbA-trnK-UUU_p5 CAAAACGCCAAACCATCCAA GAAAGCCGTGTGCAATGAAA 45.0 45.0 57.8 57.9 415-428 bp 1.0 85.9