primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnH-GUG-psbA_p1 trnH-GUG-psbA 1 GATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 20 20 55.0 55.0 58.1 58.3 0.0 0.0 0.465225 76 482 377-584 1.000000 82.9 trnH-GUG-psbA_p2 trnH-GUG-psbA 2 CAATCCACTGCCTTGATCCA CCGTGCTAACCTTGGTATGG 20 20 50.0 55.0 57.9 58.3 0.0 0.0 0.469587 76 482 391-598 1.000000 82.8 trnH-GUG-psbA_p3 trnH-GUG-psbA 3 AATCCACTGCCTTGATCCAC CCGTGCTAACCTTGGTATGG 20 20 50.0 55.0 57.9 58.3 0.0 0.0 0.471094 76 482 390-597 1.000000 82.8 trnH-GUG-psbA_p4 trnH-GUG-psbA 4 ACAATCCACTGCCTTGATCC CCGTGCTAACCTTGGTATGG 20 20 50.0 55.0 57.9 58.3 0.0 0.0 0.471094 76 482 392-599 1.000000 82.7 trnH-GUG-psbA_p5 trnH-GUG-psbA 5 GATCCACTTGGCTACATCCG ACCGTGCTAACCTTGGTATG 20 20 55.0 50.0 58.1 57.6 0.0 0.0 0.525510 76 482 378-585 1.000000 82.4 psbA-trnK-UUU_p1 psbA-trnK-UUU 1 ACGCCAAACCATCCAATGTA CTAGTTCCGGGTTCGAATCC 20 20 45.0 55.0 57.8 57.8 0.0 18.0 0.428611 1545 1748 326-339 1.000000 86.9 psbA-trnK-UUU_p2 psbA-trnK-UUU 2 TAAAGACGGTTTTCGGTGCT GAAAGCCGTGTGCAATGAAA 20 20 45.0 45.0 57.8 57.9 0.0 0.0 0.376342 1545 1748 393-406 1.000000 86.3 psbA-trnK-UUU_p3 psbA-trnK-UUU 3 ACGCCAAACCATCCAATGTA GAAAGCCGTGTGCAATGAAA 20 20 45.0 45.0 57.8 57.9 0.0 0.0 0.340872 1545 1748 411-424 1.000000 86.2 psbA-trnK-UUU_p4 psbA-trnK-UUU 4 AGGGATCATCAAAACGCCAA GAAAGCCGTGTGCAATGAAA 20 20 45.0 45.0 57.8 57.9 0.0 0.0 0.347116 1545 1748 424-437 1.000000 85.9 psbA-trnK-UUU_p5 psbA-trnK-UUU 5 CAAAACGCCAAACCATCCAA GAAAGCCGTGTGCAATGAAA 20 20 45.0 45.0 57.8 57.9 0.0 0.0 0.363955 1545 1748 415-428 1.000000 85.9 trnK-UUU-rps16_p1 trnK-UUU-rps16 1 CCGATGGTATGGACGAATCC TTCCTTGAAAAAGGCGCTCA 20 20 55.0 45.0 57.9 58.3 0.0 7.6 0.393931 4346 5165 683-1017 1.000000 83.5 trnK-UUU-rps16_p2 trnK-UUU-rps16 2 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 20 20 50.0 45.0 58.2 58.3 20.3 7.6 0.482065 4346 5165 622-956 1.000000 82.9 trnK-UUU-rps16_p3 trnK-UUU-rps16 3 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 20 20 50.0 45.0 58.2 58.3 20.3 7.6 0.482065 4346 5165 621-955 1.000000 82.9 trnK-UUU-rps16_p4 trnK-UUU-rps16 4 TCCTAGCCGCACTTAAAAGC TTCCTTGAAAAAGGCGCTCA 20 20 50.0 45.0 58.3 58.3 0.0 7.6 0.579483 4346 5165 637-971 1.000000 82.1 trnK-UUU-rps16_p5 trnK-UUU-rps16 5 CCGCACTTAAAAGCCGAGTA TTCCTTGAAAAAGGCGCTCA 20 20 50.0 45.0 58.3 58.3 5.3 7.6 0.596451 4346 5165 631-965 1.000000 82.0 rps16_p1 rps16 1 AGCAAGAAAATAAGTAGAGCAAGAA GGTTCGAAATTAGAGGAATGT 25 21 32.0 38.1 57.0 53.6 0.0 0.0 11.359965 5166 6328 1268-1302 0.962963 43.2 rps16_p2 rps16 2 AGCAAGAAAATAAGTAGAGCAAGA GGTTCGAAATTAGAGGAATGT 24 21 33.3 38.1 56.4 53.6 0.0 0.0 10.998939 5166 6328 1284-1302 0.925926 40.2 rps16_p3 rps16 3 AGCAAGAAAATAAGTAGAGCAAGA GGTTCGAAATTAGAGGAATGTT 24 22 33.3 36.4 56.4 54.5 0.0 0.0 11.158700 5166 6328 1284-1302 0.925926 40.2 rps16_p4 rps16 4 GCAAGAAAATAAGTAGAGCAAGA GGTTCGAAATTAGAGGAATGT 23 21 34.8 38.1 55.0 53.6 0.0 0.0 11.374371 5166 6328 1283-1301 0.925926 40.2 rps16_p5 rps16 5 AGCAAGAAAATAAGTAGAGCAAG GGTTCGAAATTAGAGGAATGT 23 21 34.8 38.1 55.0 53.6 0.0 0.0 11.386049 5166 6328 1284-1302 0.925926 40.2 rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 AACGGATCGTGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 57.9 58.1 7.8 16.9 0.156387 6329 7349 1061-1112 1.000000 86.9 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 20 20 45.0 55.0 58.1 58.1 8.1 16.9 0.215425 6329 7349 1044-1095 1.000000 86.5 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 CAACGGATCGTGTCCTTCAA GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.2 58.1 7.8 16.9 0.285346 6329 7349 1062-1113 1.000000 85.9 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 20 20 55.0 55.0 58.4 58.1 7.8 16.9 0.445953 6329 7349 1059-1110 1.000000 84.6 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 20 20 55.0 55.0 58.4 58.1 2.9 16.9 0.445953 6329 7349 1058-1109 1.000000 84.6 psbM-trnD-GUC_p1 psbM-trnD-GUC 1 TTGACTGGCTGTTTTTACGT TTCAATAGGCAAGAGCACCG 20 20 40.0 50.0 55.8 58.3 0.0 0.0 2.443634 10837 11484 795-821 1.000000 65.2 psbM-trnD-GUC_p2 psbM-trnD-GUC 2 TTTGACTGGCTGTTTTTACGT TTCAATAGGCAAGAGCACCG 21 20 38.1 50.0 56.6 58.3 0.0 0.0 2.671066 10837 11484 796-822 1.000000 63.3 psbM-trnD-GUC_p3 psbM-trnD-GUC 3 TTTTGACTGGCTGTTTTTACGT TTCAATAGGCAAGAGCACCG 22 20 36.4 50.0 57.3 58.3 0.0 0.0 2.967498 10837 11484 797-823 1.000000 61.0 psbM-trnD-GUC_p4 psbM-trnD-GUC 4 TGACTGGCTGTTTTTACGTA TTCAATAGGCAAGAGCACCG 20 20 40.0 50.0 55.0 58.3 0.0 0.0 3.311539 10837 11484 794-820 1.000000 58.2 psbM-trnD-GUC_p5 psbM-trnD-GUC 5 TTGACTGGCTGTTTTTACGT GTTCAATAGGCAAGAGCACC 20 20 40.0 50.0 55.8 56.8 0.0 0.0 3.395045 10837 11484 796-822 1.000000 57.5 trnY-GUA-rpoB_p1 trnY-GUA-rpoB 1 CGAATTTACAGTCCGTCCCC AGAAAGCTCCTCTGCCAAAC 20 20 55.0 50.0 58.4 58.1 0.0 0.0 0.448558 11756 12842 1152-1214 1.000000 82.8 trnY-GUA-rpoB_p2 trnY-GUA-rpoB 2 ACGAATTTACAGTCCGTCCC AGAAAGCTCCTCTGCCAAAC 20 20 50.0 50.0 57.6 58.1 0.0 0.0 0.472235 11756 12842 1153-1215 1.000000 82.7 trnY-GUA-rpoB_p3 trnY-GUA-rpoB 3 CGAATTTACAGTCCGTCCCC CTCTGCCAAACCCTGATCAA 20 20 55.0 50.0 58.4 57.8 0.0 0.0 0.556518 11756 12842 1143-1205 1.000000 82.0 trnY-GUA-rpoB_p4 trnY-GUA-rpoB 4 ATTGACAAATTGTTGGGCCG AGAAAGCTCCTCTGCCAAAC 20 20 45.0 50.0 57.5 58.1 0.0 0.0 0.551045 11756 12842 1206-1268 1.000000 81.9 trnY-GUA-rpoB_p5 trnY-GUA-rpoB 5 ACGAATTTACAGTCCGTCCC CTCTGCCAAACCCTGATCAA 20 20 50.0 50.0 57.6 57.8 0.0 0.0 0.580195 11756 12842 1144-1206 1.000000 81.8 trnT-GGU-psbD_p1 trnT-GGU-psbD 1 GTAATCCCATGGTAACGCGT GAAACGGTCCCTACGTAACC 20 20 50.0 55.0 58.1 58.0 5.6 5.0 0.059677 31051 32225 1276-1309 1.000000 87.1 trnT-GGU-psbD_p2 trnT-GGU-psbD 2 GTAATCCCATGGTAACGCGT GGACCAGCCTACAAAAACGA 20 20 50.0 50.0 58.1 58.1 5.6 0.0 0.169082 31051 32225 1294-1327 1.000000 86.2 trnT-GGU-psbD_p3 trnT-GGU-psbD 3 AGTAATCCCATGGTAACGCG GAAACGGTCCCTACGTAACC 20 20 50.0 55.0 57.8 58.0 11.7 5.0 0.238008 31051 32225 1277-1310 1.000000 85.7 trnT-GGU-psbD_p4 trnT-GGU-psbD 4 ATCCCATGGTAACGCGTAAG GAAACGGTCCCTACGTAACC 20 20 50.0 55.0 57.8 58.0 14.5 5.0 0.238008 31051 32225 1273-1306 1.000000 85.7 trnT-GGU-psbD_p5 trnT-GGU-psbD 5 GTAATCCCATGGTAACGCGT ACAAAAACGAAACGGTCCCT 20 20 50.0 45.0 58.1 58.2 5.6 0.0 0.302038 31051 32225 1284-1317 0.962963 82.2 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.137364 46310 46820 561-601 1.000000 83.8 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 20 20 55.0 50.0 57.9 58.1 0.0 0.0 0.202173 46310 46820 569-609 1.000000 83.2 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 20 20 50.0 50.0 57.8 58.0 15.5 0.0 0.245117 46310 46820 593-633 1.000000 82.7 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 20 20 50.0 50.0 57.8 58.1 15.5 0.0 0.309926 46310 46820 601-641 1.000000 82.1 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 20 20 55.0 50.0 57.9 58.2 0.0 10.2 0.372548 46310 46820 575-615 1.000000 81.8 accD-psaI_p1 accD-psaI 1 TTCCAGAAGGGCTTATTCGA AAAGAAGCCATTGCGATTGC 20 20 45.0 45.0 56.2 58.0 0.0 1.6 1.761018 58101 58829 891-915 1.000000 73.0 accD-psaI_p2 accD-psaI 2 TTCCAGAAGGGCTTATTCGA GCGATTGCCGGAAAAACTAG 20 20 45.0 50.0 56.2 57.8 0.0 5.0 1.934649 58101 58829 879-903 1.000000 71.7 accD-psaI_p3 accD-psaI 3 TCCAGAAGGGCTTATTCGAT AAAGAAGCCATTGCGATTGC 20 20 45.0 45.0 56.0 58.0 0.0 1.6 1.996346 58101 58829 890-914 1.000000 71.1 accD-psaI_p4 accD-psaI 4 TTCCAGAAGGGCTTATTCGA AGGCCTACTAAAGGGACCAA 20 20 45.0 50.0 56.2 57.6 0.0 1.3 2.114850 58101 58829 861-885 1.000000 70.3 accD-psaI_p5 accD-psaI 5 GCGGCTGAGTATTTATTCCAG AAAGAAGCCATTGCGATTGC 21 20 47.6 45.0 56.8 58.0 0.0 1.6 2.157784 58101 58829 906-930 1.000000 69.7 petA-psbJ_p1 petA-psbJ 1 ACGTGTACAAGGCCTTTTGT GGTTCATATTCCGGGTTGGG 20 20 45.0 55.0 58.0 58.3 17.6 0.0 0.359439 62660 63455 929-963 1.000000 84.5 petA-psbJ_p2 petA-psbJ 2 GGCCTTTTGTTCTTCTTCGC GGTTCATATTCCGGGTTGGG 20 20 50.0 55.0 57.9 58.3 0.0 0.0 0.364316 62660 63455 919-953 1.000000 84.5 petA-psbJ_p3 petA-psbJ 3 ACGTGTACAAGGCCTTTTGT CATATTCCGGGTTGGGTTCA 20 20 45.0 50.0 58.0 57.6 17.6 0.0 0.475546 62660 63455 925-959 1.000000 83.6 petA-psbJ_p4 petA-psbJ 4 ACGTGTACAAGGCCTTTTGT TGGTTCATATTCCGGGTTGG 20 20 45.0 50.0 58.0 57.6 17.6 0.0 0.475546 62660 63455 930-964 1.000000 83.6 petA-psbJ_p5 petA-psbJ 5 ACGTGTACAAGGCCTTTTGT GTTCATATTCCGGGTTGGGT 20 20 45.0 50.0 58.0 57.6 17.6 0.0 0.477250 62660 63455 928-962 1.000000 83.6 rpl16-rps3_p1 rpl16-rps3 1 CAAGTGCCTGAAGAGCGTAT GAGGGTAGGGTTCCACTACA 20 20 50.0 55.0 58.0 57.8 0.0 6.0 0.258618 81316 82531 1409-1448 0.962963 82.3 rpl16-rps3_p2 rpl16-rps3 2 GGGTTCAAGTGCCTGAAGAG GAGGGTAGGGTTCCACTACA 20 20 55.0 55.0 58.5 57.8 0.0 6.0 0.714753 81316 82531 1414-1453 1.000000 81.7 rpl16-rps3_p3 rpl16-rps3 3 CAAGTGCCTGAAGAGCGTAT AGAGGGTAGGGTTCCACTAC 20 20 50.0 55.0 58.0 57.5 0.0 0.0 0.562388 81316 82531 1410-1449 0.962963 79.9 rpl16-rps3_p4 rpl16-rps3 4 CAAGTGCCTGAAGAGCGTAT AGGGTAGGGTTCCACTACAA 20 20 50.0 50.0 58.0 57.3 0.0 6.0 0.743165 81316 82531 1408-1447 0.962963 78.4 rpl16-rps3_p5 rpl16-rps3 5 CAAGTGCCTGAAGAGCGTAT GAGAGGGTAGGGTTCCACTA 20 20 50.0 55.0 58.0 57.2 0.0 0.0 0.858381 81316 82531 1411-1450 0.962963 77.5 rrn5-trnR-ACG_p1 rrn5-trnR-ACG 1 GGTGACGATACTGTAGGGGA CCACGTGCTCTAATCCTCTG 20 20 55.0 55.0 57.9 57.8 0.0 10.9 0.278878 106780 107062 340-368 1.000000 85.7 rrn5-trnR-ACG_p2 rrn5-trnR-ACG 2 ATTCTGGTGTCCTAGGCGTA CCACGTGCTCTAATCCTCTG 20 20 50.0 55.0 57.8 57.8 3.0 10.9 0.370474 106780 107062 408-436 1.000000 84.3 rrn5-trnR-ACG_p3 rrn5-trnR-ACG 3 GTAGAGGAACCACACCAACC CCACGTGCTCTAATCCTCTG 20 20 55.0 55.0 57.8 57.8 0.0 10.9 0.396339 106780 107062 391-419 1.000000 84.3 rrn5-trnR-ACG_p4 rrn5-trnR-ACG 4 CGTAGAGGAACCACACCAAC CCACGTGCTCTAATCCTCTG 20 20 55.0 55.0 58.2 57.8 0.0 10.9 0.423901 106780 107062 392-420 1.000000 84.0 rrn5-trnR-ACG_p5 rrn5-trnR-ACG 5 TGTCCTAGGCGTAGAGGAAC CCACGTGCTCTAATCCTCTG 20 20 55.0 55.0 58.2 57.8 14.9 10.9 0.455715 106780 107062 401-429 1.000000 83.7 trnR-ACG-trnN-GUU_p1 trnR-ACG-trnN-GUU 1 CAGAGGATTAGAGCACGTGG CCGTTGTTCCTCAGTAGCTC 20 20 55.0 55.0 57.8 58.0 18.9 0.0 0.218238 107137 107601 605-606 1.000000 79.8 trnR-ACG-trnN-GUU_p2 trnR-ACG-trnN-GUU 2 TTTGAGGGTACTTCCGGGAA CCGTTGTTCCTCAGTAGCTC 20 20 50.0 55.0 58.3 58.0 9.5 0.0 0.270214 107137 107601 659-660 1.000000 79.3 trnR-ACG-trnN-GUU_p3 trnR-ACG-trnN-GUU 3 TTTTGAGGGTACTTCCGGGA CCGTTGTTCCTCAGTAGCTC 20 20 50.0 55.0 58.3 58.0 0.0 0.0 0.270214 107137 107601 660-661 1.000000 79.3 trnR-ACG-trnN-GUU_p4 trnR-ACG-trnN-GUU 4 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 20 20 55.0 50.0 57.8 58.2 18.9 18.9 0.372539 107137 107601 556-557 1.000000 78.7 trnR-ACG-trnN-GUU_p5 trnR-ACG-trnN-GUU 5 CAGAGGATTAGAGCACGTGG GGTTGAGAACGGGAATGGAA 20 20 55.0 50.0 57.8 57.8 18.9 0.0 0.410108 107137 107601 644-645 1.000000 78.2 trnL-UAG-rpl32_p1 trnL-UAG-rpl32 1 AGCGTGTCTACCGATTTCAC GGATATGGGGCGTCGTTAAA 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.251432 122623 123478 932-985 1.000000 89.4 trnL-UAG-rpl32_p2 trnL-UAG-rpl32 2 CAGCGTGTCTACCGATTTCA GGATATGGGGCGTCGTTAAA 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.252803 122623 123478 933-986 1.000000 89.3 trnL-UAG-rpl32_p3 trnL-UAG-rpl32 3 AGCGTGTCTACCGATTTCAC GGGCGTCGTTAAAAGCTTTG 20 20 50.0 50.0 58.0 58.3 0.0 13.5 0.326006 122623 123478 925-978 1.000000 88.8 trnL-UAG-rpl32_p4 trnL-UAG-rpl32 4 CAGCGTGTCTACCGATTTCA GGGCGTCGTTAAAAGCTTTG 20 20 50.0 50.0 58.0 58.3 0.0 13.5 0.327376 122623 123478 926-979 1.000000 88.8 trnL-UAG-rpl32_p5 trnL-UAG-rpl32 5 TCCTAAGAGCAGCGTGTCTA GGATATGGGGCGTCGTTAAA 20 20 50.0 50.0 57.9 57.8 0.0 0.0 0.367879 122623 123478 942-995 1.000000 88.4 rpl32-ndhF_p1 rpl32-ndhF 1 TTTAACGACGCCCCATATCC CCGTCGGGAAAAGAAGAAGT 20 20 50.0 50.0 57.8 57.8 0.0 2.3 0.408921 123644 124706 1171-1246 1.000000 86.3 rpl32-ndhF_p2 rpl32-ndhF 2 CAAAGCTTTTAACGACGCCC CCGTCGGGAAAAGAAGAAGT 20 20 50.0 50.0 58.3 57.8 9.5 2.3 0.483494 123644 124706 1178-1253 1.000000 85.7 rpl32-ndhF_p3 rpl32-ndhF 3 CGCCCCATATCCTTTCCTTT CCGTCGGGAAAAGAAGAAGT 20 20 50.0 50.0 57.6 57.8 0.0 2.3 0.530291 123644 124706 1163-1238 1.000000 85.3 rpl32-ndhF_p4 rpl32-ndhF 4 ACAAAGCTTTTAACGACGCC CCGTCGGGAAAAGAAGAAGT 20 20 45.0 50.0 57.6 57.8 9.5 2.3 0.559864 123644 124706 1179-1254 1.000000 85.0 rpl32-ndhF_p5 rpl32-ndhF 5 TAACGACGCCCCATATCCTT CCGTCGGGAAAAGAAGAAGT 20 20 50.0 50.0 58.6 57.8 0.0 2.3 0.749075 123644 124706 1169-1244 1.000000 83.6 ndhF-ycf1_p1 ndhF-ycf1 1 GGGTGGACGCATTTCTTCTT GTTGGTTGGATAATTGGTCACA 20 22 50.0 40.9 58.5 56.9 0.0 0.0 3.594195 126933 126974 173-243 1.000000 60.0 ndhF-ycf1_p2 ndhF-ycf1 2 GGGTGGACGCATTTCTTCTT TGTTGGTTGGATAATTGGTCAC 20 22 50.0 40.9 58.5 56.9 0.0 0.0 3.594195 126933 126974 174-244 1.000000 60.0 ndhF-ycf1_p3 ndhF-ycf1 3 GGGTGGACGCATTTCTTCTT TTGTTGGTTGGATAATTGGTCAC 20 23 50.0 39.1 58.5 57.5 0.0 0.0 3.926304 126933 126974 175-245 1.000000 57.3 ndhF-ycf1_p4 ndhF-ycf1 4 GGGTGGACGCATTTCTTCTT TGGTTGGATAATTGGTCACA 20 20 50.0 40.0 58.5 54.5 0.0 0.0 3.938477 126933 126974 171-241 1.000000 57.2 ndhF-ycf1_p5 ndhF-ycf1 5 GGGTGGACGCATTTCTTCTT TGTTGGTTGGATAATTGGTCACA 20 23 50.0 39.1 58.5 58.5 0.0 0.0 3.990877 126933 126974 174-244 1.000000 56.8