Hotspot

ndhF

SSC · 112897-115137 · 2241 bp

Back to Biancaea

Pi 0.00892

MarkerSeek score 47.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2241
variable_sites: 20
pi: 0.00892
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGTTTCGATGCAACAACCAA	TCCTGCTTACTTGGTCGTTT	40.0	45.0	56.8	57.1	2974 bp	0.5	42.7
ndhF_p2	ATGTTTCGATGCAACAACCA	TCCTGCTTACTTGGTCGTTT	40.0	45.0	56.5	57.1	2975 bp	0.5	40.9
ndhF_p3	TGTTTCGATGCAACAACCAA	ATCCTGCTTACTTGGTCGTT	40.0	45.0	56.8	56.8	2975 bp	0.5	40.9
ndhF_p4	ATGTTTCGATGCAACAACCA	ATCCTGCTTACTTGGTCGTT	40.0	45.0	56.5	56.8	2976 bp	0.5	39.1
ndhF_p5	TGTTTCGATGCAACAACCAAT	TCCTGCTTACTTGGTCGTTT	38.1	45.0	57.3	57.1	2974 bp	0.5	38.8