Hotspot

ndhD-psaC

SSC · 119431-119538 · 108 bp

Back to Biancaea

Pi 0.0648

MarkerSeek score 72.30

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 108
variable_sites: 7
pi: 0.0648
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	ACTACAACTATTGTTAACCAAGGA	AAACAACTCGAAGCATGGGT	33.3	45.0	55.7	58.0	193-194 bp	1.0	42.9
ndhD-psaC_p2	ACAACTATTGTTAACCAAGGA	AAACAACTCGAAGCATGGGT	33.3	45.0	52.7	58.0	190-191 bp	1.0	42.9
ndhD-psaC_p3	ACAACTATTGTTAACCAAGGAA	AAACAACTCGAAGCATGGGT	31.8	45.0	53.6	58.0	190-191 bp	1.0	42.9
ndhD-psaC_p4	ACTACAACTATTGTTAACCAAGGA	GGCATGAAACAACTCGAAGC	33.3	50.0	55.7	58.0	199-200 bp	1.0	42.8
ndhD-psaC_p5	ACAACTATTGTTAACCAAGGA	GGCATGAAACAACTCGAAGC	33.3	50.0	52.7	58.0	196-197 bp	1.0	42.8