Hotspot

rps11-rpl36

LSC · 81960-82112 · 153 bp

Back to Biancaea

Pi 0.0458

MarkerSeek score 68.60

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 153
variable_sites: 7
pi: 0.0458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGTCCATTTCTACGTGAACCA	CGTAGACGCGGACGAATTAT	47.6	50.0	58.3	58.0	260-262 bp	1.0	71.4
rps11-rpl36_p2	CGTCCATTTCTACGTGAACC	CGTAGACGCGGACGAATTAT	50.0	50.0	56.6	58.0	260-262 bp	1.0	70.4
rps11-rpl36_p3	CGTGAACCAATACGTCCATT	CGTAGACGCGGACGAATTAT	45.0	50.0	56.2	58.0	272-274 bp	1.0	67.1
rps11-rpl36_p4	CGTGAACCAATACGTCCATTTC	CGTAGACGCGGACGAATTAT	45.5	50.0	58.0	58.0	272-274 bp	1.0	65.8
rps11-rpl36_p5	CGTGAACCAATACGTCCATTT	CGTAGACGCGGACGAATTAT	42.9	50.0	56.9	58.0	272-274 bp	1.0	65.1