Hotspot

trnP-UGG-psaJ

LSC · 69046-69465 · 420 bp

Back to Biancaea

Pi 0.0311

MarkerSeek score 64.00

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 420
variable_sites: 13
pi: 0.0311
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	539-571 bp	1.0	80.0
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	538-570 bp	1.0	80.0
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	550-582 bp	1.0	79.9
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	549-581 bp	1.0	79.9
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	552-584 bp	1.0	79.9