Hotspot

psbE-petL

LSC · 67327-68186 · 860 bp

Back to Biancaea

Pi 0.0118

MarkerSeek score 64.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 860
variable_sites: 10
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GTCGTATCTTGCTCAGACCG	45.0	55.0	58.0	58.2	1063-1065 bp	1.0	80.1
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCG	50.0	55.0	57.9	58.2	1052-1054 bp	1.0	79.8
psbE-petL_p3	TGACAAATAACCAACCCGCA	TCGTATCTTGCTCAGACCGA	45.0	50.0	58.0	58.3	1062-1064 bp	1.0	79.4
psbE-petL_p4	CAACCCGCAATGAATAGGGA	TCGTATCTTGCTCAGACCGA	50.0	50.0	57.9	58.3	1051-1053 bp	1.0	79.2
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCG	50.0	55.0	57.8	58.2	975-977 bp	1.0	79.1