Hotspot

rbcL-accD

LSC · 59521-60447 · 927 bp

Back to Biancaea

Pi 0.0266
MarkerSeek score 65.20
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
927
variable_sites
24
pi
0.0266
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 TTTGAATTCCCAGCAATGGA CGGGTCTTCACTTACACTGG 40.0 55.0 55.5 57.9 1077-1445 bp 1.0 62.4
rbcL-accD_p2 TGAATTCCCAGCAATGGATACT CGGGTCTTCACTTACACTGG 40.9 55.0 57.5 57.9 1075-1443 bp 1.0 62.3
rbcL-accD_p3 TTGAATTCCCAGCAATGGAT CGGGTCTTCACTTACACTGG 40.0 55.0 55.2 57.9 1076-1444 bp 1.0 60.5
rbcL-accD_p4 ATTTGAATTCCCAGCAATGGA CGGGTCTTCACTTACACTGG 38.1 55.0 56.1 57.9 1078-1446 bp 1.0 59.1
rbcL-accD_p5 TTTGAATTCCCAGCAATGGAT CGGGTCTTCACTTACACTGG 38.1 55.0 56.1 57.9 1077-1445 bp 1.0 59.1