Hotspot

rps16-trnQ-UUG

LSC · 6350-6948 · 599 bp

Back to Biancaea

Pi 0.0389

MarkerSeek score 75.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 599
variable_sites: 23
pi: 0.0389
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GTCGAATAGAAATGGGGCGT	50.0	50.0	58.0	58.1	739-953 bp	1.0	82.5
rps16-trnQ-UUG_p2	GTCTTTCAAGTCGCACGTTG	TCGAATAGAAATGGGGCGTG	50.0	50.0	58.0	58.1	738-952 bp	1.0	82.5
rps16-trnQ-UUG_p3	GTCTTTCAAGTCGCACGTTG	TGTCGAATAGAAATGGGGCG	50.0	50.0	58.0	58.1	740-954 bp	1.0	82.5
rps16-trnQ-UUG_p4	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	679-893 bp	1.0	82.5
rps16-trnQ-UUG_p5	ATGTCTTTCAAGTCGCACGT	GTCGAATAGAAATGGGGCGT	45.0	50.0	58.1	58.1	741-955 bp	1.0	81.4