Hotspot

ycf15-ycf2

IRa · 142495-142592 · 98 bp

Back to Betonica

Pi 0.0108

MarkerSeek score 63.40

Primer available yes

Label ycf15-ycf2

Feature Metadata

label_name: ycf15-ycf2
feature_type: igs
region: IRa
length_bp: 98
variable_sites: 1
pi: 0.0108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-ycf2_p1	GTTCGGGCAGTTCATACCAT	ATGGCTTTTCCCGGATGAAA	50.0	45.0	58.0	57.8	257-262 bp	1.0	79.7
ycf15-ycf2_p2	GTTCGGGCAGTTCATACCAT	TGGCTTTTCCCGGATGAAAT	50.0	45.0	58.0	57.8	256-261 bp	1.0	79.7
ycf15-ycf2_p3	TGGTTGTTCGCTGTTCAAGA	ATGGCTTTTCCCGGATGAAA	45.0	45.0	58.0	57.8	283-288 bp	1.0	79.6
ycf15-ycf2_p4	TGGTTGTTCGCTGTTCAAGA	TGGCTTTTCCCGGATGAAAT	45.0	45.0	58.0	57.8	282-287 bp	1.0	79.6
ycf15-ycf2_p5	TGGTTGTTCGCTGTTCAAGA	AAGATGGCTTTTCCCGGATG	45.0	50.0	58.0	58.2	286-291 bp	1.0	79.6