Hotspot

ccsA-ndhD

SSC · 113186-113366 · 181 bp

Back to Betonica

Pi 0.0444

MarkerSeek score 63.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 181
variable_sites: 8
pi: 0.0444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CGGCCCTTTTATGTGGATCA	TCAGTAGCTTTTCGATGGCC	50.0	50.0	57.9	58.0	1082 bp	1.0	80.9
ccsA-ndhD_p2	CGGCCCTTTTATGTGGATCA	CAGTAGCTTTTCGATGGCCT	50.0	50.0	57.9	58.0	1081 bp	1.0	80.9
ccsA-ndhD_p3	CGGCCCTTTTATGTGGATCA	TATCTTGACGAAATGGGCGG	50.0	50.0	57.9	58.1	1137 bp	1.0	80.7
ccsA-ndhD_p4	CGGCCCTTTTATGTGGATCA	TGACGAAATGGGCGGAATAG	50.0	50.0	57.9	58.1	1132 bp	1.0	80.7
ccsA-ndhD_p5	GTTGGGACCCAAAGGAAACT	TCAGTAGCTTTTCGATGGCC	50.0	50.0	57.9	58.0	761 bp	1.0	80.7