Hotspot

ndhF-rpl32

SSC · 110645-111148 · 504 bp

Back to Betonica

Pi 0.0956

MarkerSeek score 80.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 504
variable_sites: 48
pi: 0.0956
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACGAAAGGTAAAATACACGCA	AAATGCTTTTAACGCTGCCC	38.1	45.0	56.2	57.9	641-648 bp	1.0	63.2
ndhF-rpl32_p2	ACGAAAGGTAAAATACACGCA	GCCCGATACCCCTTCTTTTT	38.1	50.0	56.2	57.9	625-632 bp	1.0	62.9
ndhF-rpl32_p3	GAACGAAAGGTAAAATACACGCA	AAATGCTTTTAACGCTGCCC	39.1	45.0	58.0	57.9	643-650 bp	1.0	61.4
ndhF-rpl32_p4	GAACGAAAGGTAAAATACACGCA	GCCCGATACCCCTTCTTTTT	39.1	50.0	58.0	57.9	627-634 bp	1.0	61.1
ndhF-rpl32_p5	AACGAAAGGTAAAATACACGCA	AAATGCTTTTAACGCTGCCC	36.4	45.0	56.9	57.9	642-649 bp	1.0	61.0