Hotspot

trnG-GCC-trnM-CAU

LSC · 36230-36373 · 144 bp

Back to Betonica

Pi 0.035
MarkerSeek score 73.20
Primer available yes
Label trnG-GCC-trnM-CAU

Feature Metadata

label_name
trnG-GCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
144
variable_sites
5
pi
0.035
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 211-212 bp 1.0 81.5
trnG-GCC-trnM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 212-213 bp 1.0 81.5
trnG-GCC-trnM-CAU_p3 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 247-248 bp 1.0 81.3
trnG-GCC-trnM-CAU_p4 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 248-249 bp 1.0 81.3
trnG-GCC-trnM-CAU_p5 ATGCGGATATGGTCGAATGG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.9 58.0 288-289 bp 1.0 80.7