Hotspot

trnT-GGU-psbD

LSC · 31440-32659 · 1220 bp

Back to Betonica

Pi 0.0254

MarkerSeek score 66.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1220
variable_sites: 31
pi: 0.0254
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1371-1373 bp	1.0	79.4
trnT-GGU-psbD_p2	ATAGTGACTCGTCCGGGAAT	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1417-1419 bp	1.0	79.3
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1374-1376 bp	1.0	79.2
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	55.0	50.0	58.1	57.6	1370-1372 bp	1.0	78.2
trnT-GGU-psbD_p5	ATAGTGACTCGTCCGGGAAT	CAAAAACGAAACGGTCCCTC	50.0	50.0	57.9	57.6	1416-1418 bp	1.0	78.1