Hotspot

rps16-trnQ-UUG

LSC · 6388-7086 · 699 bp

Back to Betonica

Pi 0.0203
MarkerSeek score 65.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
699
variable_sites
13
pi
0.0203
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CCAAGAGCACCCTTATTCCC GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.9 58.1 786-843 bp 1.0 84.2
rps16-trnQ-UUG_p2 AACGGATCGTGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 732-789 bp 1.0 84.1
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 715-772 bp 1.0 83.7
rps16-trnQ-UUG_p4 CAACGGATCGTGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 733-790 bp 1.0 83.1
rps16-trnQ-UUG_p5 AGAGCACCCTTATTCCCAGA GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 783-840 bp 1.0 82.5