Hotspot

ycf2-trnL-CAA

IRa · 115689-115807 · 119 bp

Back to Beta

Pi 0.0448
MarkerSeek score 68.60
Primer available yes
Label ycf2-trnL-CAA

Feature Metadata

label_name
ycf2-trnL-CAA
feature_type
igs
region
IRa
length_bp
119
variable_sites
8
pi
0.0448
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnL-CAA_p1 ATGGCTTTTCCCGGATGAAA AAATGGTAGACACGCGAGAC 45.0 50.0 57.8 58.0 235 bp 1.0 80.8
ycf2-trnL-CAA_p2 TGGCTTTTCCCGGATGAAAT AAATGGTAGACACGCGAGAC 45.0 50.0 57.8 58.0 234 bp 1.0 80.8
ycf2-trnL-CAA_p3 ATGGCTTTTCCCGGATGAAA TGAAATGGTAGACACGCGAG 45.0 50.0 57.8 58.0 237 bp 1.0 80.8
ycf2-trnL-CAA_p4 TGGCTTTTCCCGGATGAAAT TGAAATGGTAGACACGCGAG 45.0 50.0 57.8 58.0 236 bp 1.0 80.8
ycf2-trnL-CAA_p5 AAGATGGCTTTTCCCGGATG AAATGGTAGACACGCGAGAC 50.0 50.0 58.2 58.0 238 bp 1.0 80.8