Hotspot

psbE-petL

LSC · 88037-89280 · 1244 bp

Back to Beta

Pi 0.0204
MarkerSeek score 57.80
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1244
variable_sites
38
pi
0.0204
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 GAAACGGTAGGTACAGCCAG CTCCTGCGGGTTAGAGAAAC 55.0 55.0 58.0 58.0 2012-2016 bp 1.0 81.6
psbE-petL_p2 CATTGCGGATATTGACCCCA CTCCTGCGGGTTAGAGAAAC 50.0 55.0 58.0 58.0 2037-2041 bp 1.0 81.5
psbE-petL_p3 GAAACGGTAGGTACAGCCAG AGCGCTCTTAGTTCAGTTCG 55.0 50.0 58.0 58.0 2164-2168 bp 1.0 81.4
psbE-petL_p4 CAAGTGGTCGTGCTAGAGAC CTCCTGCGGGTTAGAGAAAC 55.0 55.0 58.0 58.0 2312-2316 bp 1.0 81.3
psbE-petL_p5 CAAGTGGTCGTGCTAGAGAC AGCGCTCTTAGTTCAGTTCG 55.0 50.0 58.0 58.0 2464-2468 bp 1.0 81.2