Hotspot

pafI-trnS-GGA

LSC · 68051-68669 · 619 bp

Back to Beta

Pi 0.0129
MarkerSeek score 62.60
Primer available yes
Label pafI-trnS-GGA

Feature Metadata

label_name
pafI-trnS-GGA
feature_type
igs
region
LSC
length_bp
619
variable_sites
12
pi
0.0129
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
pafI-trnS-GGA_p1 CATTTATCCGCGATCTAGGCA CCCTCGGTAAACAAAAGCCT 47.6 50.0 58.0 58.1 708 bp 1.0 72.3
pafI-trnS-GGA_p2 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 47.6 50.0 58.0 58.2 729 bp 1.0 71.8
pafI-trnS-GGA_p3 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTGAA 47.6 45.0 58.0 57.8 679 bp 1.0 71.5
pafI-trnS-GGA_p4 TGGCCACAATTGAAAAGGTC CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.8 58.1 744 bp 1.0 71.0
pafI-trnS-GGA_p5 TGGCCACAATTGAAAAGGTC ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.8 58.2 765 bp 1.0 70.5