Hotspot

atpF-atpH

LSC · 36869-37292 · 424 bp

Pi 0.0189

MarkerSeek score 60.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 424
variable_sites: 12
pi: 0.0189
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGACCCAAGAAAACGA	GCAGAGGGAAAAATACGAGGT	50.0	47.6	58.0	58.0	580-590 bp	1.0	73.5
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GCAGAGGGAAAAATACGAGGT	50.0	47.6	57.9	58.0	607-617 bp	1.0	72.8
atpF-atpH_p3	CCAGTGACCCAAGAAAACGA	AGCAGAGGGAAAAATACGAGG	50.0	47.6	58.0	57.7	581-591 bp	1.0	71.5
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	AGCAGAGGGAAAAATACGAGG	50.0	47.6	57.9	57.7	608-618 bp	1.0	70.7
atpF-atpH_p5	CCAGTGACCCAAGAAAACGA	CGAAGCAGAGGGAAAAATACG	50.0	47.6	58.0	57.3	584-594 bp	1.0	68.3