Hotspot

rps16-trnQ-UUG

LSC · 30192-30788 · 597 bp

Back to Beta

Pi 0.0124
MarkerSeek score 62.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
597
variable_sites
11
pi
0.0124
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CCACAACGGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 691-697 bp 1.0 80.3
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 670-676 bp 1.0 80.2
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 681-687 bp 1.0 79.6
rps16-trnQ-UUG_p4 CCACAACGGATCATGTCCTT TAAGGCATCGGGTTTTGGTC 50.0 50.0 57.9 58.2 721-727 bp 1.0 79.5
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TAAGGCATCGGGTTTTGGTC 45.0 50.0 58.1 58.2 700-706 bp 1.0 79.3