Hotspot

ndhA

SSC · 120752-123083 · 2332 bp

Back to Berlinia

Pi 0.00645

MarkerSeek score 47.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2332
variable_sites: 15
pi: 0.00645
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGGGAGAAGTTGATCGTTGA	ACCCAGTTACCATAAGGAACA	45.0	42.9	56.5	56.3	2451-2454 bp	1.0	46.7
ndhA_p2	TGGGAGAAGTTGATCGTTGAA	ACCCAGTTACCATAAGGAACA	42.9	42.9	57.2	56.3	2451-2454 bp	1.0	44.7
ndhA_p3	TGGGAGAAGTTGATCGTTGA	AACCCAGTTACCATAAGGAACA	45.0	40.9	56.5	57.0	2452-2455 bp	1.0	44.7
ndhA_p4	TGGGAGAAGTTGATCGTTGA	TGAACCCAGTTACCATAAGGA	45.0	42.9	56.5	56.0	2454-2457 bp	1.0	44.5
ndhA_p5	TGGGAGAAGTTGATCGTTGA	GAACCCAGTTACCATAAGGAACA	45.0	43.5	56.5	58.1	2453-2456 bp	1.0	44.0