Hotspot

trnN-GUU-trnR-ACG

IRa · 134667-135369 · 703 bp

Back to Berlinia

Pi 0.0101

MarkerSeek score 66.20

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 703
variable_sites: 7
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	874-885 bp	1.0	79.2
trnN-GUU-trnR-ACG_p2	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	797-808 bp	1.0	79.0
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	784-795 bp	1.0	78.9
trnN-GUU-trnR-ACG_p4	TGGGTTGAGAACGGGAATTG	GAACAGATCCAGTGGAGACG	50.0	55.0	58.1	57.7	911-922 bp	1.0	78.6
trnN-GUU-trnR-ACG_p5	GGGTTGAGAACGGGAATTGA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	873-884 bp	1.0	78.4