Hotspot

trnR-ACG-trnN-GUU

IRb · 112259-112961 · 703 bp

Back to Berlinia

Pi 0.0101

MarkerSeek score 66.20

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 703
variable_sites: 7
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	874-885 bp	1.0	79.2
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	797-808 bp	1.0	79.0
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	784-795 bp	1.0	78.9
trnR-ACG-trnN-GUU_p4	GAACAGATCCAGTGGAGACG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.7	58.1	911-922 bp	1.0	78.6
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	GGGTTGAGAACGGGAATTGA	55.0	50.0	57.8	57.8	873-884 bp	1.0	78.4