Hotspot

petL-petG

LSC · 69047-69238 · 192 bp

Back to Berlinia

Pi 0.016

MarkerSeek score 64.20

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 192
variable_sites: 3
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	ATTGGGCTGAGCAAGATACG	GTTCCAACTGATCACCACGT	50.0	50.0	58.0	58.1	327-331 bp	1.0	80.6
petL-petG_p2	ATTGGGCTGAGCAAGATACG	TTCCAACTGATCACCACGTC	50.0	50.0	58.0	57.8	326-330 bp	1.0	80.3
petL-petG_p3	ATTGGGCTGAGCAAGATACG	AGTTCCAACTGATCACCACG	50.0	50.0	58.0	57.8	328-332 bp	1.0	80.3
petL-petG_p4	GGCTGAGCAAGATACGACTT	GTTCCAACTGATCACCACGT	50.0	50.0	57.7	58.1	323-327 bp	1.0	78.6
petL-petG_p5	GGCTGAGCAAGATACGACTT	TTCCAACTGATCACCACGTC	50.0	50.0	57.7	57.8	322-326 bp	1.0	78.3