Hotspot

psbL-psbF

LSC · 67220-67241 · 22 bp

Back to Berlinia

Pi 0.0455

MarkerSeek score 67.20

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 22
variable_sites: 1
pi: 0.0455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	ATAACCCCCAGTAGAGGCTG	TCAGCAATGCAGTTCATCCA	55.0	45.0	58.2	57.8	116 bp	1.0	77.5
psbL-psbF_p2	ATAACCCCCAGTAGAGGCTG	GCAATGCAGTTCATCCAACG	55.0	50.0	58.2	58.4	113 bp	1.0	76.1
psbL-psbF_p3	AATAACCCCCAGTAGAGGCT	TCAGCAATGCAGTTCATCCA	50.0	45.0	57.4	57.8	117 bp	1.0	74.3
psbL-psbF_p4	AATAACCCCCAGTAGAGGCT	GCAATGCAGTTCATCCAACG	50.0	50.0	57.4	58.4	114 bp	1.0	73.0
psbL-psbF_p5	ATAACCCCCAGTAGAGGCTG	CAGCAATGCAGTTCATCCAA	55.0	45.0	58.2	57.0	115 bp	1.0	71.0