Hotspot

petA-psbJ

LSC · 66025-66851 · 827 bp

Back to Berlinia

Pi 0.00971

MarkerSeek score 49.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 827
variable_sites: 8
pi: 0.00971
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCCGGGACCAGAACTTTTTG	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1636-1670 bp	1.0	80.6
petA-psbJ_p2	TCCGGGACCAGAACTTTTTG	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1635-1669 bp	1.0	80.6
petA-psbJ_p3	TCCGGGACCAGAACTTTTTG	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	1738-1772 bp	1.0	80.5
petA-psbJ_p4	TCCGGGACCAGAACTTTTTG	GATCTTTTTAGGAGGCGCCA	50.0	50.0	58.0	58.0	1592-1626 bp	1.0	80.3
petA-psbJ_p5	TCCGGGACCAGAACTTTTTG	GAGCCGACAAGGAATTCCAT	50.0	50.0	58.0	58.0	1660-1694 bp	1.0	80.3