Hotspot

trnF-GAA-ndhJ

LSC · 51595-52347 · 753 bp

Back to Berlinia

Pi 0.007

MarkerSeek score 67.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 753
variable_sites: 5
pi: 0.007
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATGCCCGAAAGTTGGATAGG	55.0	50.0	58.0	57.7	872-904 bp	1.0	81.2
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	836-868 bp	1.0	80.9
trnF-GAA-ndhJ_p3	AGAATGGTCGGGATAGCTCA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	57.9	57.7	881-913 bp	1.0	80.4
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	ATGCCCGAAAGTTGGATAGG	55.0	50.0	57.9	57.7	860-892 bp	1.0	80.1
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	GCCCGAAAGTTGGATAGGAT	55.0	50.0	58.0	57.4	870-902 bp	1.0	78.8