Hotspot

trnG-GCC-trnfM-CAU

LSC · 39583-39739 · 157 bp

Back to Berlinia

Pi 0.0423

MarkerSeek score 76.00

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 157
variable_sites: 6
pi: 0.0423
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	220-235 bp	1.0	84.4
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	218-233 bp	1.0	83.3
trnG-GCC-trnfM-CAU_p3	GGGTTCAATTCCCGCTATCC	CTTGAGGTCACGGGTTCAAA	55.0	50.0	58.4	58.0	199-214 bp	1.0	82.9
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	241-256 bp	1.0	82.8
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	240-255 bp	1.0	82.8