Hotspot

psbZ-trnG-GCC

LSC · 38878-39511 · 634 bp

Back to Berlinia

Pi 0.0259

MarkerSeek score 68.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 634
variable_sites: 16
pi: 0.0259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	784-788 bp	1.0	81.8
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	787-791 bp	1.0	80.2
psbZ-trnG-GCC_p3	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	780-784 bp	1.0	80.1
psbZ-trnG-GCC_p4	CTTCTCCTGATGGTTGGTCG	GGATAGCGGGAATTGAACCC	55.0	55.0	58.0	58.4	805-809 bp	1.0	79.9
psbZ-trnG-GCC_p5	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	785-789 bp	1.0	79.8