Hotspot

psbC-trnS-UGA

LSC · 37984-38217 · 234 bp

Back to Berlinia

Pi 0.0427

MarkerSeek score 70.50

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 234
variable_sites: 10
pi: 0.0427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	AGCTGCAGCAGGATTTGAAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.4	58.0	399 bp	1.0	78.8
psbC-trnS-UGA_p2	AGCTGCAGCAGGATTTGAAA	GCTGAGTGGTTGATAGCTCC	45.0	55.0	58.4	58.1	391 bp	1.0	78.6
psbC-trnS-UGA_p3	AGCTGCAGCAGGATTTGAAA	GGCTGAGTGGTTGATAGCTC	45.0	55.0	58.4	58.1	392 bp	1.0	78.6
psbC-trnS-UGA_p4	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	384 bp	1.0	78.6
psbC-trnS-UGA_p5	TAGGTCATTTATGGCACGCG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.4	58.0	433 bp	1.0	78.3