Hotspot

petN-psbM

LSC · 30173-31421 · 1249 bp

Back to Berlinia

Pi 0.00728

MarkerSeek score 63.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1249
variable_sites: 9
pi: 0.00728
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1405-1412 bp	1.0	68.8
petN-psbM_p2	CCCTCGTAGTATGGGGAAGA	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.6	57.6	1354-1361 bp	1.0	67.0
petN-psbM_p3	TCCCTCGTAGTATGGGGAAG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.6	57.6	1355-1362 bp	1.0	67.0
petN-psbM_p4	GGGGGCATAATTCACATGGA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1427-1434 bp	1.0	66.9
petN-psbM_p5	AGGGGGCATAATTCACATGG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1428-1435 bp	1.0	66.9