Hotspot

trnR-UCU-atpA

LSC · 10384-10797 · 414 bp

Back to Berlinia

Pi 0.0154

MarkerSeek score 68.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 414
variable_sites: 6
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CATTTACCGGGGAAGCAGAA	47.6	50.0	57.3	57.9	528-542 bp	1.0	70.1
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	AAGACATTTACCGGGGAAGC	47.6	50.0	57.3	57.9	532-546 bp	1.0	70.1
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	GGAAGCAGAAGCCCTTTTGA	47.6	50.0	57.3	58.4	518-532 bp	1.0	68.1
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ATTTACCGGGGAAGCAGAAG	47.6	50.0	57.3	57.6	527-541 bp	1.0	67.7
trnR-UCU-atpA_p5	TGGATAGGACAGAGGTCTTCT	CATTTACCGGGGAAGCAGAA	47.6	50.0	56.9	57.9	563-577 bp	1.0	67.0